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Title   정상 혈구의 마우스 단세포군 항체에 대한 수용체 ( Receptors for Murine Monoclonal Antibodies on the Normal Blood Cells )
Publicationinfo   1993 Jan; 025(02): 252-261.
Key_word   Monoclonal antibody, Blood cells, Monocyte, Fc receptor
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Abstract   Several murine monoclonal antibodies have been used as targeting agents for radioimmunoscintigraphy and radioimmunotherapy. However, there is a possibility that these antibodies bind to normal blood cells. And these bindings can cause adverse or favourable results in the radioimmunoscintigrapy and immunotherapy. In this investigation, we tried to evaluate the bindings between normal blood cells and commonly used murine monoclonal antibodies, such as IgGl, IgG2a. We used B-72.3, COL-1 as murine IgGI antibodies and 9.2.27, 96.5, 2-135, T- 10l as murine IgG2a antibodies. 1) Neither monoclonal antibodies IgG1 or IgG2a bound to normal red blood cells. 2) Monoclonal antibody IgGl wont bind to normal white blood cells. But IgG2a antibodies showed specific bindings with white blood cells. 3) Monoclonal antibody IgG2a didn't bind to T-lymphocytes. However, it baund to monocytes specifically which was confirmed by FACS analysis. 4) Bindings of 125I-9.2.27(IgG2a) to monocytes were inhibited by addition of other IgG2a anti- bodies or Fc fragment. This binding was not inhibited by adding F(ab')2 fragments of IgG2a. These findings suggested that binding of IgG2a to monocyte was related with Fc receptor of monocytes. 5) From the Scatchard analysis, the association constant(Ka) and receptor number of monocytes were derived as 1.2~1.5 x 10(9)1/mole, and 2.2~3,1 x 10(4)/cell, respectively. 6) After binding of 125I-9.2.27 to monocytes, these cells were cultured until 24 hours. Cell bound 125I-9.2.27 gradually disappeared from monocytes. And progressive accumulation of radioactivity in the culture supernatants occurred throughout the culture period.
저 자   정준기(June Key Chung),이명철(Myung Chul Lee),고창순(Chang Soo Koh)